Organoid and Cell Culture Core (OCCC)
The Organoid and Cell Culture Core (OCCC) generates models that replicate the structural and functional complexity of the gastrointestinal tract and liver. The core offers a wide range of frozen organoids and will additionally develop cell-based, and tissue engineering-centric models of the gastrointestinal tract and liver that will facilitate research into normal regulatory processes, as well as mechanisms of digestive diseases and response to therapy.
- To provide a repository of digestive disease-specific human and mouse organoids.
- To provide a repository of digestive cell lines whose identify is confirmed and that are annotated for passage and mycoplasma negative; as well as advanced cell culture platforms, protocols and technologies
- To provide multi-core workflows in collaboration with other CU-DLDRC cores, allowing for functional analysis of transriptional regulators through Cas9 and dCas9-transgenic organoids and organoid- and cell-based drug and CRISPR screens
- To provide CU-DLDRC investigators and their labs with training on cell and organoid culture models
The anticipated start date for OCCC is March 2022. An updated will be provided in December ’21 through this website as well as an OCCC seminar in spring ’22. Once the core starts, a detailed list of services and fee schedule will be provided through this website and the iLab system.
OCCC already works with CU-DLDRC investigators to expand organoids and protocols – please contact core directors Hiroshi Nakagawa and Kelley Yan for specific projects and needs.